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Manufacturing Complexity: Peptide-antibody conjugates pose challenges for large-scale production

Subsequently, the tissue fragments were inoculated into T75 culture flasks and, once the tissue adhered, the medium was replaced with complete medium (MEM, 20% FBS, 1% PS, 2 g/mL epidermal growth factor EGF, 25 ng/mL fibroblast growth factor FGF) to facilitate cell migration

This finding indicates that the lateral rotational movement of the KAR ligand-binding domains is critical for complete channel closure and stabilization of the fully desensitized receptor

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van den Broek, S