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The linear regression lines of the plot of PKH26 ( x -axis) versus the other fluorescent protein ( y -axis) of the EVs from the single transfected cells (e.g., GFP-MIM I-BAR in Halo7-Rac1 transfected EVs) were used to identify another fluorescence, where the standard deviation (SD) of the signals from the regression line in another fluorescence channels was used as the cut-off line for the positive particles of another fluorescence

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