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Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

Moreover, current animal models are limited in their ability to fully replicate the complex pathological state of hypertension and lung cancer comorbidity, which hampers experimental design and interpretation

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NF-B Activation and Chronic Inflammation GSH normally maintains NF-B in its inactive state by preserving the redox status of IB kinase within immune cells

Mossmann, D