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The extracellular domain of DPP-4, which harbors its catalytic site, often gets released from the membrane and circulates in plasma

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Optical rotations were recorded at 20 C on a Perkin-Elmer 343 digital polarimeter at 589 nm with a cell path length of 10 cm

After harmonized the extracted data, a screening process was implemented to exclude IVs that were closely associated with outcomes ( P 0.05)

The absorbance was measured at 570 nm using a microplate reader to determine the cell viability