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Plasmid construction GSH1 was amplified from genomic DNA of CEN.PK 113-7D using High-Fidelity DNA Polymerase (Thermo Fisher Scientific) with primers GSH1_SalI_fw (5 ATAA GTCGAC ATGGGACTCTTAGCTTTGGG) and GSH1_KpnI_rev (5 GCAT GGTACC TTAACATTTGCTTTCTATTGAAG) and cloned into the integrative plasmid YIplac211[37] harboring the promoter TDH3p, the terminator CYC1t and a functional copy of URA3

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Stimulation of Phosphoinositide Hydrolysis by Trans-(+/-)-Acpd Is Greatly Enhanced When Astrocytes Are Cultured in a Serum-Free Defined Medium