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Integrative phosphatidylcholine metabolism through phospholipase A 2 in rats with chronic kidney disease

Key Takeaway: Consistency matters more than precision timing

Upon reaching sufficient density, the cells were transferred to a low-protein BSA-free medium (LPM, Biological Industries) complemented with 1 mM sodium pyruvate (Biological Industries), 2 mM glutamine (Biological Industries), nonessential amino acids (Biological Industries), 100 /ml penicillin (Biological Industries) and 0.1 mg/ml streptomycin (Biological Industries)

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While this method bypasses the GI tract and generally ensures more reliable absorption, it is not without its drawbacks