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Using real-world data The study has been published in Nature Health and investigated data from 410,198 Reddit posts by 67,008 users, between May 2019 and June 2025

GLP-1 stands for glucagon-like peptide-1 , a hormone that your body naturally releases from the gut after you eat

Then germ-free mice were used to verify whether high-tryptophan-induced anti-tumor effect of liver cancer depends on gut microbiota

The sections were first incubated overnight in peroxidase-conjugated sheep anti-digoxigenin antibody Fab fragments, and then the Glp1r hybridization signal was amplified using the TSA Plus DIG Kit (Akoya Biosciences, Cat# NEL748E001KT) for 30 min, applying the DIG amplification reagent at 1:500 dilution in 0.05 M Tris (pH 7.6) containing 0.01% H 2 O 2