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Cells were grown at 37 C to an optical density at 600 nm (OD 600 ) of 0.70.8 in lysogeny broth (LPS solution LB-05) medium, and then 0.5 mM of isopropyl-1-thio--D-galactopyranoside (LPS solution IPTG025) was added

Medical-grade travel coolers designed for temperature-sensitive medications work best, but even a simple insulated lunch bag with a few small ice packs will maintain refrigeration-like temperatures for 8-12 hours, which covers most travel scenarios

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