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To study the intracellular levels of ROS and GSH in viable cells, the samples were incubated with 10 M CellTracker Blue (Thermo Fisher Scientific, Barcelona, Spain) and 10 M CM-H 2 DCFDA (Thermo Fisher Scientific, Barcelona, Spain) for 30 min at 38.5 C, followed by 0.5 M PI staining

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Transformation of oil-producing fungus, Mortierella alpina 1S-4, using Zeocin, and application to arachidonic acid production

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