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Establish a rotation schedule and stick to it

McGuireDKAlexanderJHJohansenOEPerkovicVRosenstockJCooperMEet al

doi: 10.1016/j.preghy.2021.01.001 115

The four sub-amounts sum back to the total: 1,667 + 333 + 333 + 333 = 2,666 mcg, rounding to ~2,667 mcg

Yeast cells (5 10 5 /ml at the start) were routinely cultured in YPD medium (1% yeast extract, 2% soybean peptone and 2% glucose) (Sigma-Aldrich) for 18 h at 30C with shaking (170 rpm) using an orbital rotary shaker MaxQ 6000 (ThermoFisher Scientific, Waltham, MA, United States)