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The sequencing reads were aligned to the reference human genome (GRCh38) using Twist Exome 2.0 bed file (Twist Biosciences, USA) with MiniMap2 [26] and alignments were corrected by Medaka ( Variant calling was performed using Clair3-Trio [27], generating variant call format (VCF) files

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These are normal functions that happen every time you eat