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Atherosclerotic CVD manifest as heart attacks and strokes, is responsible for up to 85 per cent of cardiovascular deaths [1]

Co-localisation of SNAP/FLAG-hGLP-1R with cholesterol in the same cells using the fluorescent D4H*-mCherry probe to label plasma membrane cholesterol at the cytosolic leaflet (32) corroborated our previous results as we measured a significant decrease in co-localisation between GLP-1R V229A and cholesterol under vehicle conditions compared to WT GLP-1R (Figure 3E-G)

This was, evolutionarily speaking, a way to survive times of famine

2-DE separates proteins into multiple spots based on their isoelectric point and molecular weight, whereas Western blotting (WB) detects the total amount of the entire protein

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