A fraction (100 l/110 5 cells) of the cell suspension was incubated with 5 l Annexin V conjugated to FITC and 5 l propidium iodide (PI) for 15 mins at 25C in the dark
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Next, we explore the role of Siglec-8 and sialic acid interactions in regulating eosinophil activity and ROS in the gut, including how dietary influences (Neu5Ac vs
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