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DNA samples harvested from electroporations were digested with BslI and ClaI (NEB) to prepare ends for ligation to the sequencing adapter, then mixed with 20 pM duplexed adapter and 3000 Units of T7 DNA ligase (NEB)

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doi:10.3389/fendo.2019.00388

These secondary effects, such as gut microbiome dysbiosis, reduced diversity, and barrier compromise, may offset the anti-inflammatory effects of these medications

To read the article for yourself, click here: The conversation around peptides is evolving, and we'll be paying close attention to next week's Pharmacy Compounding Advisory Committee meeting