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As expected, the strictly conserved Asn419 hTrxR1 appears to be the most important residue for aligning the flexible C-terminal arm during catalysis, because a simultaneous mutation of all three guiding bar residues (Trp407 hTrxR1 , Asn418 hTrxR1 , and Asn419 hTrxR1 ) led only to a minor increase in hTrx1- and DTNB-reduction activity of hTrxR1 compared to the mutation of Asn419 hTrxR1 alone (Table 2)

However, patients should report any new or worsening abdominal pain to their healthcare provider, regardless of dosing patterns