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The resulting supernatant was centrifuged at 9,500 g for 10 minutes and the mitochondrial pellet was resuspended in a minimal amount of isolation buffer
Human tissue was obtained from the National Institutes of Health NeuroBioBank at the University of Miami Brain Endowment Bank and University of Pittsburgh Brain Tissue Donation Program
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The divergent hepatic responses to L-carnitine suggest stage-dependent differences in hepatic metabolic response that may reflect disease severity, potentially arising from differences in metabolic flexibility, carnitine transport, mitochondrial function, or baseline saturation of hepatic acetylcarnitine
Understanding potential adverse effects enables informed decision-making and appropriate medical monitoring
