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Muscle growth (hypertrophy) occurs when resistance training creates microscopic damage to muscle fibers

To generate G3BP-tdTomato knock-in U2OS cells that stably express WT or mutant forms of ASPL, HEK293T (CRL-3216) cells were first transfected with the N174 lentiviral vector (Addgene, #31822) containing different ASPL cDNAs along with packaging vectors PSPAX2 and PMD2.g using Lipofectamine 2000 (Thermo Fisher Scientific)

The underlying genetic flaw responsible for disease inheritance in these two families was found to be mutations in the CARD14 gene, according to next-generation sequencing

The 2025 meta-analysis by Alfalasi and colleagues, published via PMC, synthesized data from 16 studies and 6,098 total participants