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Bulk RNA-seq and bioinformatics analysis Bulk RNA-seq samples were sequenced using the TruSeq dual-index sequencing primers on Illumina HiSeq 2000, 2500 or MiSeq (50 bp single-end sequencing) platforms

Efficiency in extracorporeal membrane oxygenation-cellular deposits on polymethylpentene membranes increase resistance to blood flow and reduce gas exchange capacity

For immunofluorescence, the next day, the secondary antibody and 4,6-diamino-2-phenylpyridine (DAPI) nuclear stain were applied to the paraffin sections, which were then mounted with an anti-quenching solution

The copper peptide addresses certain aspects of follicle health while other treatments address different factors

Results revealed a higher mtDNA copy number in Epitalon-treated oocytes compared to untreated aged counterparts, further supporting the idea that Epitalon maintains mitochondrial integrity during oocyte aging