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Tissue and single-cell qRT-PCR RNA from nodose ganglia was extracted using the RNeasy Micro Plus Kit (Qiagen)

What the evidence also shows is that the medication alone is not the whole answer

Similarly, berberine treatment can significantly reduce the oxidative stress in diabetic rat hippocampus and suppress GFAP immunoreactive astrocytes ( Studies have found that the combined treatment of DOX and berberine can significantly counteract the increase in acetylcholinesterase activity, oxidative stress, the decrease in glutathione content and CAT activity, and the increase in GFAP, NF-B and caspase-3 induced by DOX (Zhang et al., 2012)

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