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Isolated mitochondria were washed twice with basic STE buffer (without 0.1% fatty acidfree BSA), resuspended in basic STE buffer and protein concentration of mitochondrial proteins was determined by the bicinchoninic acid (BCA) assay using BSA as a standard

10.1002/jlb.3a0620-348rr 59 GureevA

Values are the averages of three independent experiments performed in triplicateSEM

The mechanisms by which smoking induces vascular endothelial dysfunction and the effects of smoking cessation on vascular endothelial function will be reviewed

Vitamin B12 Deficiency Symptoms Deficiency may result in various disturbances such as hematological, neurological, and psychiatric problems, observed as the following symptoms: Feeling tired/weak or confused Tingling or numbness in fingers and toes Constipation, balance issues Depression and cognitive issues A swollen, inflamed tongue Anemia Deficiency symptoms tend to manifest gradually over a few years, and it is recommended to check blood serum levels for early detection [7]