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Then, primary antibody binding was detected after incubating sections with a horseradish peroxidase conjugated-secondary antibody (polyclonal anti-goat or anti-rabbit IgG HRP, R&D System, Minneapolis, MN, USA) for 2.5 h, followed by a 20 min exposure to the HRP substrate 3-amino-9-ethylcarbazole (AEC)

Plant Gene 28:100342

This can be achieved using antiangiogenic agents which promote vascular normalization [28, 29]

It is necessary to determine pharmacodynamics, i.e., the binding affinity to the GABA receptor, as well

By combining the two ingredients, you may be able to achieve the desired lightening effects while using less hydroquinone, lowering the risk of side effects