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In some specific protocols, yes, but physiological saline (0.9% NaCl) contains no preservative, so it has shorter solution stability, similar to sterile water
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Vitamin B12 deficiencies can be caused by many factors, such as older age, gastrointestinal conditions, and vegan or vegetarian diets
Lakowicz and Gregorio Weber reported that despite their common location in the interior of the protein matrix and the apparent inaccessibility to solvent, no tryptophan residues were excluded from quenching by oxygen (which is an ideal quencher of the fluorescence of singlet excited states, as every collision with an excited fluorophore results in quenching), indicating that proteins, in general, undergo rapid structural fluctuations on the nanosecond time scale which permit diffusion of oxygen (Lakowicz and Weber, 1973)