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Antagonising glucagon receptors (GCGRs) with modified glucagon analogues, small molecules or antisense oligonucleotides has also been problematic because of the risk of hypoglycaemia and adverse effects on liver function, including steatosis and an increase in LDL-cholesterol

These patterns consistently lead to poor outcomes

Lane 16: trypsin digestion of 6mGLP-1 for 15, 30, 45, 60, 90, 120 min at 37C, respectively

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