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the shRNA was transfected into cells using the lipofectamine method described above

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Soluble fiber supplementation (such as psyllium) may help by binding bile acids and improving stool consistency

Nausea, transient vomiting, and altered bowel habits reflect receptor engagement in the gut and the slower gastric emptying that produces satiety

While the studies presented in this review have made use of preclinical in vivo studies to explore the mechanisms underlying the biological mechanisms, an interesting avenue potentially aiding to gain further insight into the processes involved the effects of nutritional strategies in the context of ES and their relevance in the context of human biology, are humanised in vitro models