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Addressing response rates, a commenter stated that approach used to calculate the 7 percent response rate was equivalent to the American Association of Public Opinion Research (AAPOR) standard response rate calculation as it relates to whether delivered but unopened email invitations should be included in the denominator of the response rates

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The mild increase in DNA fragmentation at 200 g/mL is consistent with the elevated necrosis at that concentration: membrane disruption exposes chromatin to extracellular nucleases and divalent metal ions, accelerating fragmentation independently of ROS 51

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