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IR in the brain impairs glucose metabolism, which is critical for the provision of neuronal energy and the maintenance of synaptic function

Measurement of single-myocyte excitability, contractility, and Ca 2+ transients Myocytes were enzymatically isolated as described previously ( 2+ transients (loaded with FURA-2AM)

The mammalian sirtuins (Sirt1--Sirt7) participate in a number of cellular and physiological functions such as gene silencing, apoptosis, mitochondrial function, energy homeostasis, and longevity [237].Transgenic mice with overexpression of the sirt1 gene demonstrate decreased lipid-induced inflammation, improved glucose tolerance, and protection from hepatic steatosis when fed a high-fat diet [238]

doi:10.1007/s11845-021-02754-5 6