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UBE2D2 and Ub were isolated using cation-exchange chromatography and eluted with either 50 mM HEPES pH 7, 250 mM NaCl, 5 mM DTT or 25 mM sodium acetate pH 4.5, 250 mM NaCl, respectively

1998;105(1B):31S38S Singh S, Khajuria A, Taneja, SC, et al

However, a meta-analysis reported that the use of NAC showed no significant improvement in pregnancy rate, serum LH level, fasting insulin, and LH/follicle-stimulating hormone (FSH) ratio despite that the BMI and total testosterone were significantly reduced (138)

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