After the mice were sacrificed, for liver, pancreas, adipose, colon tissue samples (G6Pase, GLP-1, TNF-, IL-6 and MCP-1 detection), the tissue was homogenized with PBS, then the homogenate was centrifuged at 4 C for 10 min at 4000 g, supernatant was used for ELISA analysis
Refrigeration and sharps containers arent needed with oral GLP-1s
This approach isn't practical for clinical dosing protocols but is standard in laboratory settings where batch consistency matters more than convenience
If youre new to meal prep, start by making just a couple of make-ahead meals each week
Questions to Ask Your Healthcare Provider Which GLP-1 side effects should I report immediately