After blocking with TBST containing 5% nonfat milk for 1 h, the membranes were incubated with GPX4 (rabbit, 22 kDa, ab125066, 1:5,000, Abcam), 4-hydroxynonenal (4-HNE) (rabbit, 1776 kDa, ab46545, 1:2,500, Abcam), acyl-CoA synthetase long-chain family member 4 (ACSL4) (mouse, 75 kDa, sc-365230, 1:1,000, Santa Cruz), solute carrier family 7 member 11 (SLC7A11) (rabbit, 55 kDa, ab175186, 1:5,000, Abcam), Anti-5 Lipoxygenase (5-LOX) (rabbit, 78 kDa, ab169755, 1:1,000, Abcam) and -actin (mouse, 43 kDa, A5441, 1:5,000, Sigma) overnight at 4C
Effects on antioxidant enzyme activities SOD acts as the first line of defense against ROS-induced toxicity by catalyzing superoxide radicals to form oxygen and H 2 O 2 88
Repair restores homeostatic signaling
Neutrophil depletion protects against murine acetaminophen hepatotoxicity
Lack of long-term safety data: As a relatively new compound, the long-term safety profile of Dihexa is not yet well-established