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Cells were resuspended in 2% FACS buffer containing a primary histone acetylation antibody (Table S12) and incubated O/N at 4C on a roller

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We need to be very mindful of any products that may not be proven and therefore may have no effect or unknown negative consequences

By investigating these compounds in both conventional and niosomal formulations, this research will contribute to the development of more effective strategies to counteract ATZ-induced reproductive toxicity, ultimately improving male fertility outcomes