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Clean the rubber tops of both the MOTS-c vial and the BAC water vial with an alcohol swab Draw 0.5 mL of bacteriostatic water into a 3 mL syringe Inject the BAC water slowly into the MOTS-c vial, angling the stream down the side wall rather than directly onto the powder Gently swirl the vial until the powder is fully dissolved, do not shake Draw 25 units from the reconstituted vial into an insulin syringe for injection Store the reconstituted vial in the refrigerator between doses Injection Site and Technique The injection is subcutaneous, into the abdomen

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Flags theoretical pathologic angiogenesis potential: PMC12446177 Evidence Summary Preclinical: IBD/colitis models: High remission rates, restored mucosal architecture, improved barrier function Tendon repair: Accelerated healing, improved tensile strength, reduced fibrosis Neurovascular: Sciatic nerve protection, enhanced blood vessel growth in nerve tissue Human data (emerging): Interstitial cystitis pilot (2024, n=12): 10/12 complete resolution, 2/12 at 80% improvement IV safety study (2025, n=2): no adverse effects on cardiac, hepatic, renal, or glucose biomarkers Knee pain retrospective (2021, n=17): 91.6% reported significant improvement No large-scale controlled trials published Safety: No immunosuppression or hormonal disruption in available data 2025 systematic review (HSS Journal): recommends caution due to limited high-quality clinical evidence 2025 narrative review: flags theoretical pathologic angiogenesis risk Stable in gastric acid (oral administration viable) No observed toxicity at therapeutic doses in published reports Foundational Reviews Sikiric P, Seiwerth S, et al

Yes restoring SCFA production, mucosal integrity, and microbial diversity helps maintain natural GLP-1 signaling without medication

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