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Excess secondary antibody was washed off by 3 consecutive 10-min rinses with PBST

In innate immunity, Clostridium modulates macrophage polarization (shifting from pro-inflammatory M1 to anti-inflammatory M2), induces tolerogenic dendritic cell phenotypes to suppress excessive antigen presentation, regulates inflammatory body activity and neutrophil hyperactivation, and modulates ILC subsets to reinforce mucosal defense

At higher pH levels, cysteine can form disulfide bonds with other cysteine residues in the same peptide or even in neighboring peptide molecules

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