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pastoris (GAP), and a galactose-based GAL1 promoter of S

Co-expression of membrane-tethered EX4 and free EX4 peptide hormone Codon-optimized sequences encoding EX4 peptide hormone were inserted in frame into the vector expressing 5xNG linker followed by a MYC tag, a G plus 9xNG linker, a single-pass TMD, and a 3X-FLAG tag

Moreover, given that we detected an increases in the levels of FXR-antagonistic BAs, but did not observe an elevation in the levels of TGR5-agonistic BAs and cAMP, we then speculate that FXR-mediated pathway rather than TGR5-mediated pathway was critically involved in the regulation of GLP-1 by T3 treatment in mice

Methods 21 , 12161221 (2024)