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Plasmid construction and yeast transformation All plasmids were constructed using conventional restriction enzyme-mediated cloning methods

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In contrast, the bulkier Y37 rAmy in rAmy-bound AMY 2 R prevents equivalent interactions of N35 rAmy with RAMP2, and instead the bulkier Y37 rAmy forms interactions with E101 RAMP2 and F111 RAMP2 stabilising the peptide at the CTR/RAMP ECD interface in the bypass conformation

It also helps regenerate glutathione in the body after it has been used to fight off free radicals

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