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Diluted pooled samples were loaded on an Illumina MiSeq and sequenced using a 500-cycle MiSeq Reagent Kit v3

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For example, in alveolar macrophages, GLP-1 receptor agonism suppressed activation of the NLRP3 inflammasome and reduced production of IL-1 [65], an effect in part mediated by the disruption of histone lactylation, a recognised regulator of pro-fibrotic gene expression [65]

This format provides a convenient research tool for investigations involving comparative peptide pharmacology, concurrent evaluation of multiple signaling mechanisms, and studies examining interactions between distinct peptide-associated regulatory pathways