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Mutation of cysteine 347 of GLP-1R to lysine or arginine transforms the receptor into a G protein-biased receptor and rescues the loss-of-function of the truncated peptides GPCRs have been viewed as allosteric machines [33] because many allosteric modulators have been identified binding outside the orthosteric pocket of the physiological activators to modulate endogenous agonist binding and function

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