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Specifically, we suggest that the benzyl portion of the isobenzopyrrole-1,3-dione moiety of CP17 might be modified to provide groups able to make polar or charge interactions with the carbonyl oxygen of VP1-Ile89 or the carboxylate of the sidechain of VP1 Asp114 (Fig

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The immunoprecipitates were mixed with protein A-agarose beads (sc2003, Santa Cruz) and rotated for 2 h at 4 C

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