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The M1 phenotype is often associated with disease, resulting in production and secretion of pro-inflammatory cytokines (i.e., tumor necrosis factor-alpha (TNF), interleukin-1 (IL-1), and IL-6), chemokines, and ROS to neutralize pathogens and increase cell recruitment to the site of inflammation

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High-throughput metabolomics: Isocratic and gradient mass spectrometry-based methods

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Generally safe, but individuals with underlying health conditions should consult a physician before use