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MS-based proteomics Hypothalamic tissue was powdered and lysed (lysis buffer 50 mM Tris, 4% SDS buffer, pH 8.5) using BeatBox homogenizer (PreOmics)

It caused the neurons to undergo apoptosis, or programmed cell death

VLDL affects HDL metabolism

"The effect of microbial colonization on the host proteome varies by gastrointestinal location." The ISME journal (2015)

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