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In contrast to the class Ia diferric tyrosyl radical (Fe III 2 -Y) cofactor, which can self-assemble and mature from Fe II 2 -NrdF and molecular O 2 , generation of the Mn III 2 -Y cofactor in the class Ib enzyme requires the reduced form of a flavoprotein, NrdI hq , and O 2 to create superoxide anion for active site assembly and oxidative maturation