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In addition, studies on murine hepatic mitochondria have shown that DZN inhibits state III respiration, which is driven by glutamate and malate, substrates of complex I of the respiratory chain, as well as succinate, a substrate of complex II [10]

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For mitochondrial activity, the cells were incubated with 200 mM MitoTracker Deep Red (Thermo Fisher Scientific, Barcelona, Spain) for 20 min at 38.5 C in the dark and then stained with 10 M YO-PRO-1 and 0.5 M PI