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At a determined period of time (e.g., 6 hours, 12 hours, 1 day, 2 days, 3 days, 4 days, 5 days, 6 days, 7 days, and 14 days for testing the pharmacokinetics of a formulation or composition over a 1 or 2 week period), the test animal is euthanized and the inner ear removed and tested for the presence of the otic agent

The rules are genuinely in flux right now, the FDA is reviewing several of these compounds, and the list of whats allowed keeps shifting, but as of today, the trendy ones largely sit outside what any licensed physician can legally and responsibly prescribe

Apart from hydrolases, two individual degradative gene clusters each for PA and PCA metabolism were detected (Additional file 8: Table S3, Fig