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In neuronal networks not expressing ChR2, after the end of experiments Propidium Iodide (PI) (Invitrogen) (1:1000) was added to each well and incubated for 15 min at room temperature (RT) to detect dead cells (red)

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Angiotensin II (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe) binds at the AT 1 receptor subtype and mediates systemic blood pressure, body water/electrolyte balance and influences cerebral blood flow, cerebroprotection, seizure, stress and depression

Peptides stimulate the pituitary gland and hypothalamus to produce its own HGH, so they keep the pituitarys negative feedback loop involved, which keeps the pituitary gland from burning out

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