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3b) and NP2 had an average hydrodynamic diameter of 116 nm and a PDI of 0.07 (Supplementary Fig

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The gradient used was as follows (at a flow rate of 0.5 ml min 1 ): 0% solvent B for 1.6 min, followed by an increase to 30% solvent B over 2.4 min, a further increase to 90% solvent B over 0.5 min, a hold at 90% solvent B for 0.5 min, a return to 0% solvent B over 0.1 min and equilibration at 0% solvent B for 1.4 min, for a total run time of 6.5 min per sample