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Habituation to the operator and the testing environment At 56 wk of age in the week before behavioural testing, mice were habituated to the experimenter over three consecutive days through a gradual increase in handling in order to reduce handling-related anxiety (Deacon, 2006)

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TIRF Microscopy was done at room temperature (21 C) using a Nikon Eclipse Ti2 microscope equipped with 405 nm and 488 nm lasers and an ORCA-Fusion BT Digital CMOS camera installed in the Advanced Light and Fluorescent Microscopy (ALFM) Facility in CSSB Hamburg (DESY, Hamburg, Germany) using NIS Elements (v5.42.04)