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Total Cholesterol (TC) 0.5ml of working reagent was added to a set of clean labeled test tubes and two of the test tubes were labeled standard 1 and 2 while another was labeled blank.0.1ml of homogenate was added to the approximately labeled test tube .mixed and incubated for 30 minutes at 37, the absorbance of the sample and standard were read against the reagent blank at the wavelength of 546nm
B12 deficiency takes 13 years to show symptoms by then, nerve damage may be permanent
Canon, F., Maillard, M
Secretary of Education
2.5 Serum liver function parameters For evaluating the liver function, serum levels of alanine transaminase (ALT)/aspartate transaminase (AST), and alkaline phosphatase (ALP) were measured using the Bio-Diagnostic colorimetric kits (Giza, Egypt)
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