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Thyroid hormone mediates these effects at the transcriptional and post-translational levels and via autophagy

provided PCs to T2D mice (75, 150, and 300 mg/kg) via continuous gavage for 4 weeks, documenting improvements in intestinal barrier integrity by restoring morphology and raising the expression of tight junction proteins, including Zonula occludens-1 (ZO-1), Claudin-1, and occludin

Lastly, we ensured that the sample comprised only individuals with NAFLD and type 2 diabetes using an exhaustive list of quality checks and diagnosis codes, including but not limited to the exclusion of alcohol-related disorders, alcohol abuse, alcohol dependence and alcohol-related liver diseases

A prior check list should be to have realistic approach and expectation