Primary antibodies used for detecting shelterin subunits were custom rabbit anti-TRF1 (1:5,000, Thermo Scientific), mouse anti-TRF2 antibody (1:10,000, Abcam ab13579) and mouse anti-TIN2 antibody (1:10,000, Novus Biologicals 59B388.1)
[25] Gamma-carboxyglutamate proteins [edit] The following human Gla-containing proteins ("Gla proteins") have been characterized to the level of primary structure: blood coagulation factors II (prothrombin), VII, IX, and X, anticoagulant protein C and protein S, and the factor X-targeting protein Z
This, combined with heightened inflammation and fibrosis, is believed to contribute to an increased risk of hepatocellular carcinoma
The manufacturer sets a discharge current, monitors voltage, temperature, and capacity
Oligo dT primers and primers for real-time PCR were obtained from Integrated DNA Technologies (IDT, Coralville, IA, USA)