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Regulation of CD8 + T cell-mediated immune responses by amino acid metabolism Glutamine Glutamine is deaminated to form glutamate and -ketoglutarate (-KG), which supports anaplerosis of the TCA cycle and plays a crucial role in regulating histone methylation and chromatin accessibility (Fig

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The levels of mRNA of target genes were normalized to GAPDH using a QuantStudio 7 Real-Time PCR system (Thermo Fisher, MA, USA)

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