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Fluorescence intensities were recorded using the Synergy H1 multi-detection microplate reader (Biotek, Bad Friedrichshall, Germany) at excitation wavelengths 405 and 488 nm and emission wavelength at 525 nm at 25 C

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N-hexanoic-Tyr-Ile-(6)amino-hexanoic amide was chosen for further investigation with the expectation that it would be biologically active, metabolically stable, and BBB permeable